In Russia of 1904, Pinky and the Brain attempt to steal the Czar's crown jewels so they can take over Russia. When Pinky points out that the jewels are guarded by fierce soldiers known as Cossacks, Brain says that is no problem as a lunar eclipse will occur that night, making everywhere so dark no one can see anything, as such they can sneak into the treasury without the Cossacks' knowledge. Only one other problem stands in their way, Brian always reacts to a gong and Pinky dances whenever a bell rings.
The shot of Brain's temper growing is used in the Pinky and the Brain spin-off theme.
This is a parody of Pavlov's Dog
Ivan Pavlov did not study conditioned responses, he really just wanted some saliva to study. This goof was mentioned in the "Please Please Please Get a Life Foundation" cartoon. However, due to the constant conditioned responses he was getting, he is credited for "Pavlovian responses" in psychology and the study of behaviors.
The Warners are Rasputin's dentists when he comes down with a toothache, causing him no end of misery. When Rasputin attempts to rid the Warners by having the Tsar arrest them, his teeth fall out, rendering his hypnosis ineffective. The Tsar kicks Rasputin out and thanks the Warners for saving Russia.
The Warners claim to be professional Shriners. In actuality, the Shriners are not a career, but a men's nonprofit organization that has built several hospitals for children who are burns victims and/or in need of artificial limbs. They are more famous for the red fezes and tiny cars driven in parades to raise awareness for the hospitals.
The belief that Rasputin hypnotized the Tsar into doing his bidding was the widespread rumors that he used hypnosis to cure the Tsar's son whenever he had medical problems to cure him, which is now largely discredited.
A little girl knocking out Rasputin was a play on the term anesthesia. Dot remarks that most kids probably would not get it and tells them to ask their parents. The little girl was meant to be Anastasia, the youngest daughter of Tsar Nicholas II.
One accurate scene shown was where Rasputin was appointing ministers as he saw fit. In 1916 (when the episode took place) the First World War was on, and Tsar Nicholas II was not in his palace, but on the front lines acting as supreme commander of the army. In the Tsar's absence, he entrusted most of the decisions of day-to-day government operations to his wife, who in turn deferred much to Rasputin. As a result, Rasputin wielded enormous political clout, hiring and firing ministers willy-nilly.